Smart MS 5020
Mass spectrometer for microorganism identification
System Features
Manual adjustment of the target to a point target
Laser power settings
Quick analysis of spectrogram data
Real-time assessment of the analyzer's status
Multiple use of the target plate
Calibration based on a single E. coli strain
The Smart MS 5020 mass spectrometer is
Multifunctionality
Quality
Optimized software provides information on the microscopic morphology of cells and a description of colony morphology on the culture medium, which assists in verifying the results.
Components from top global manufacturers:
Laser: Lasertechnik Berlin GmbH (Germany)
Ion detector: Hamamatsu (Japan)
Vacuum pump: Edwards (England)
Practicality
Cost-effectiveness
Sample preparation without requiring extensive extraction and protein accumulation processes
Rapid results compared to traditional methods and PCR — identification of 96 samples in less than 25 minutes.
Accessibility
Informative value
The modular library contains data on over 5,000 species of microorganisms. It is designed to improve the efficiency and accuracy of detection.
Conducting research does not require specialists with narrow expertise in mass spectrometry; any specialist can use the analyzer.
Workflow Procedure
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The detector. Fixes the previously amplified flow of electrons of the time-of-flight column. The ionic strength of the sample is converted to a digital voltage signal and collected by a high-speed data acquisition card.
Time-of-flight column. Accelerates ions with different mass-to-charge ratios in a magnetic field.
Laser. Consists of a pulsed laser generator, an energy regulator, and a focusing lens. Automatic adjustment via the laser software. Emits ultraviolet light with a wavelength of 337 nm.
Vacuum pump. The system includes two pumps. The vacuum pump creates a vacuum at the sample loading point. The molecular pump operates within the time-of-flight column to enhance its performance.
Sample loading area and ion supply unit. It consists of three parts: a target sample platform (which moves the sample into the appropriate position), a vacuum interlock (which isolates the vacuum from the external environment), and ion optics (which accelerate ions from the source and direct them into the time-of-flight column).
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Data acquisition scheme
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Ions are formed in the time-of-flight column and move toward the detector within a magnetic field.
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The detector registers the arrival of an ion and displays this in a spectrogram.
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The spectrogram records arrival data and displays it according to mass (P1 > P2 > P3), while the height of the peak depends on the quantity of identical primitive molecules.
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Reagent usage scheme
Selection of a pure culture colony for further processing
Selection of the appropriate type of reagent for disrupting bacterial cell walls (Type I for Gram-positive bacteria, Type II for all other bacteria, Type III for filamentous fungi, spores, etc.)
Adding reagent to colonies on the target plate and drying it
Addition of matrix reagent
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Mixing the matrix reagent with the sample and drying to form a co-crystal
Loading the target plate into the mass spectrometer for bacterial identification
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Technical Specification
Detection quality range
in the range of 1 Da – 500 kDa
Mass accuracy:
≤ 100 ppm (external standard), ≤ 60 ppm (internal standard)
Operating temperature
10-30° С
Relative humidity
70%
Atmospheric pressure
76-106 kPa
Device weight and dimensions
120 kg; 1150x460x690 mm
Accessories
Stainless steel target plate
A high-density coating process is used to ensure a uniform surface on the target plate and allow for repeated use.
Quality control of partitions to reduce unnecessary losses during operation
Reagent kit
Reagents for sample pretreatment; matrix reagent
Source of electrical energy
220 V, 50 Hz
